Review





Similar Products

mouse anti wtap mab  (Proteintech)
96
Proteintech mouse anti wtap mab
Mouse Anti Wtap Mab, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti wtap mab/product/Proteintech
Average 96 stars, based on 1 article reviews
mouse anti wtap mab - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
mouse anti wtap  (Proteintech)
96
Proteintech mouse anti wtap
Mouse Anti Wtap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti wtap/product/Proteintech
Average 96 stars, based on 1 article reviews
mouse anti wtap - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
mouse monoclonal anti wtap  (Proteintech)
96
Proteintech mouse monoclonal anti wtap
Mouse Monoclonal Anti Wtap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti wtap/product/Proteintech
Average 96 stars, based on 1 article reviews
mouse monoclonal anti wtap - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
mouse wtap  (Proteintech)
96
Proteintech mouse wtap
Mouse Wtap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse wtap/product/Proteintech
Average 96 stars, based on 1 article reviews
mouse wtap - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
wtap  (Proteintech)
97
Proteintech wtap
Wtap, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wtap/product/Proteintech
Average 97 stars, based on 1 article reviews
wtap - by Bioz Stars, 2026-03
97/100 stars
  Buy from Supplier
mouse anti -wtap  (Proteintech)
90
Proteintech mouse anti -wtap
<t>WTAP</t> and IGF2BP2 mediates the M6A modification <t>of</t> <t>FLOT1</t> and maintains its stability in gliomas. (A) MeRIP-qPCR indicating the m6A enrichment of FLOT1 transcripts in U251 and T98G cells. (B) RT-qPCR and (C) WB results indicating the effects of WTAP depletion on the level of FLOT1 transcript and protein in U251 and T98G cells. (D) and (E) RT-qPCR indicating the stability of FLOT1 mRNA in U251 and T98G cells upon actinomycin D (10 μg/mL) treatment. (F) Relative luciferase activity of U251 and T98G cells transfected with siNC and siWTAP. (G) RT-qPCR and (H) WB results indicating the effects of IGF2BP2 depletion on the level of FLOT1 transcript and protein in U251 and T98G cells. (I) and (J) RT-qPCR indicating the stability of FLOT1 mRNA in U251 and T98G cells upon actinomycin D (10 μg/mL) treatment. (K) Relative luciferase activity of U251 and T98G cells transfected with siNC and siWTAP. (L) RIP-qPCR indicating the enrichment of FLOT1 transcripts by IGF2BP2 immunoprecipitation in U251 and T98G cells. (M) RNA pulldown assay showing the direct interaction of FLOT1 transcripts with the IGF2BP2 protein. NS stands for no significant difference. * , P < 0.05; ** , P < 0.01; *** , P < 0.001; ns , no significant difference.
Mouse Anti Wtap, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti -wtap/product/Proteintech
Average 90 stars, based on 1 article reviews
mouse anti -wtap - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
wtap mouse monoclonal  (Proteintech)
90
Proteintech wtap mouse monoclonal
<t>WTAP</t> and IGF2BP2 mediates the M6A modification <t>of</t> <t>FLOT1</t> and maintains its stability in gliomas. (A) MeRIP-qPCR indicating the m6A enrichment of FLOT1 transcripts in U251 and T98G cells. (B) RT-qPCR and (C) WB results indicating the effects of WTAP depletion on the level of FLOT1 transcript and protein in U251 and T98G cells. (D) and (E) RT-qPCR indicating the stability of FLOT1 mRNA in U251 and T98G cells upon actinomycin D (10 μg/mL) treatment. (F) Relative luciferase activity of U251 and T98G cells transfected with siNC and siWTAP. (G) RT-qPCR and (H) WB results indicating the effects of IGF2BP2 depletion on the level of FLOT1 transcript and protein in U251 and T98G cells. (I) and (J) RT-qPCR indicating the stability of FLOT1 mRNA in U251 and T98G cells upon actinomycin D (10 μg/mL) treatment. (K) Relative luciferase activity of U251 and T98G cells transfected with siNC and siWTAP. (L) RIP-qPCR indicating the enrichment of FLOT1 transcripts by IGF2BP2 immunoprecipitation in U251 and T98G cells. (M) RNA pulldown assay showing the direct interaction of FLOT1 transcripts with the IGF2BP2 protein. NS stands for no significant difference. * , P < 0.05; ** , P < 0.01; *** , P < 0.001; ns , no significant difference.
Wtap Mouse Monoclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/wtap mouse monoclonal/product/Proteintech
Average 90 stars, based on 1 article reviews
wtap mouse monoclonal - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


WTAP and IGF2BP2 mediates the M6A modification of FLOT1 and maintains its stability in gliomas. (A) MeRIP-qPCR indicating the m6A enrichment of FLOT1 transcripts in U251 and T98G cells. (B) RT-qPCR and (C) WB results indicating the effects of WTAP depletion on the level of FLOT1 transcript and protein in U251 and T98G cells. (D) and (E) RT-qPCR indicating the stability of FLOT1 mRNA in U251 and T98G cells upon actinomycin D (10 μg/mL) treatment. (F) Relative luciferase activity of U251 and T98G cells transfected with siNC and siWTAP. (G) RT-qPCR and (H) WB results indicating the effects of IGF2BP2 depletion on the level of FLOT1 transcript and protein in U251 and T98G cells. (I) and (J) RT-qPCR indicating the stability of FLOT1 mRNA in U251 and T98G cells upon actinomycin D (10 μg/mL) treatment. (K) Relative luciferase activity of U251 and T98G cells transfected with siNC and siWTAP. (L) RIP-qPCR indicating the enrichment of FLOT1 transcripts by IGF2BP2 immunoprecipitation in U251 and T98G cells. (M) RNA pulldown assay showing the direct interaction of FLOT1 transcripts with the IGF2BP2 protein. NS stands for no significant difference. * , P < 0.05; ** , P < 0.01; *** , P < 0.001; ns , no significant difference.

Journal: Heliyon

Article Title: FLOT1, stabilized by WTAP/IGF2BP2 mediated N6-methyladenosine modification, predicts poor prognosis and promotes growth and invasion in gliomas

doi: 10.1016/j.heliyon.2023.e16280

Figure Lengend Snippet: WTAP and IGF2BP2 mediates the M6A modification of FLOT1 and maintains its stability in gliomas. (A) MeRIP-qPCR indicating the m6A enrichment of FLOT1 transcripts in U251 and T98G cells. (B) RT-qPCR and (C) WB results indicating the effects of WTAP depletion on the level of FLOT1 transcript and protein in U251 and T98G cells. (D) and (E) RT-qPCR indicating the stability of FLOT1 mRNA in U251 and T98G cells upon actinomycin D (10 μg/mL) treatment. (F) Relative luciferase activity of U251 and T98G cells transfected with siNC and siWTAP. (G) RT-qPCR and (H) WB results indicating the effects of IGF2BP2 depletion on the level of FLOT1 transcript and protein in U251 and T98G cells. (I) and (J) RT-qPCR indicating the stability of FLOT1 mRNA in U251 and T98G cells upon actinomycin D (10 μg/mL) treatment. (K) Relative luciferase activity of U251 and T98G cells transfected with siNC and siWTAP. (L) RIP-qPCR indicating the enrichment of FLOT1 transcripts by IGF2BP2 immunoprecipitation in U251 and T98G cells. (M) RNA pulldown assay showing the direct interaction of FLOT1 transcripts with the IGF2BP2 protein. NS stands for no significant difference. * , P < 0.05; ** , P < 0.01; *** , P < 0.001; ns , no significant difference.

Article Snippet: The membranes were blocked with 5% non-fat milk and incubated with the appropriate primary antibodies, including rabbit anti -FLOT1 (Abcam; Cambridge, UK), rabbit anti -IGF2BP2, mouse anti -WTAP (Proteintech; IL, USA), and rabbit anti -GAPDH (Bioworld; Nanjing, China).

Techniques: Modification, Quantitative RT-PCR, Luciferase, Activity Assay, Transfection, Immunoprecipitation

Ectopic expression of FLOT1 restores the effects of WTAP and IGF2BP2 depletion in gliomas. (A) WB results indicating the level of FLOT1, WTAP and IGF2BP2 protein in U251 and T98G cells co-transfected with siWTAP/siIGF2BP2 and FLOT1 plasmids. (B) CCK-8 assay and (C) Transwell assay indicating the growth and invasion of U251 and T98G cells co-transfected with siWTAP/siIGF2BP2 and FLOT1 plasmids. ** , P < 0.01; *** , P < 0.001.

Journal: Heliyon

Article Title: FLOT1, stabilized by WTAP/IGF2BP2 mediated N6-methyladenosine modification, predicts poor prognosis and promotes growth and invasion in gliomas

doi: 10.1016/j.heliyon.2023.e16280

Figure Lengend Snippet: Ectopic expression of FLOT1 restores the effects of WTAP and IGF2BP2 depletion in gliomas. (A) WB results indicating the level of FLOT1, WTAP and IGF2BP2 protein in U251 and T98G cells co-transfected with siWTAP/siIGF2BP2 and FLOT1 plasmids. (B) CCK-8 assay and (C) Transwell assay indicating the growth and invasion of U251 and T98G cells co-transfected with siWTAP/siIGF2BP2 and FLOT1 plasmids. ** , P < 0.01; *** , P < 0.001.

Article Snippet: The membranes were blocked with 5% non-fat milk and incubated with the appropriate primary antibodies, including rabbit anti -FLOT1 (Abcam; Cambridge, UK), rabbit anti -IGF2BP2, mouse anti -WTAP (Proteintech; IL, USA), and rabbit anti -GAPDH (Bioworld; Nanjing, China).

Techniques: Expressing, Transfection, CCK-8 Assay, Transwell Assay